Julie Cubeta, and Drs. Carl and Theresa Crozier, Henry Van Cotter, and Marc Cubeta participated in the fourth annual bioblitz at Goose Creek State Park on September 27, 2014 located on the shores of the Pamlico Sound in Washington, North Carolina (Beaufort County) to characterize the fungal biodiversity in the park. A comprehensive list of 63 fungal and 1 slime mold species found in the park during the Bioblitz was submitted to Ed Corey, Inventory Biologist with the NC Division of Parks and Recreation in December 2014. The rare species Veloporphyrellus conicus (syn. Tylopilus conicus) was found at the park and has been archived and assigned an accession number (VC 1821) in the Larry F. Grand Mycological Herbarium. We greatly acknowledge the assistance of Dr. Rytas Vilgalys, Duke University, for his help in identifying species and providing resources for DNA analyses.